Fees, Signup, and Scheduling

Fees for FY 08:

800: $9.50/hour

600: $5/hour

500: $11/hour including cryoprobe

400: $5.25/hour

The 500 is driven by an automatic sample changer.  Seven standard experiments make up the great majority of what it does.  The default parameters for those experiments and corresponding costs are (daytime rates with cryoprobe):

Experiment     # scans     time     cost @$11/hr

Proton                 16         1:05         $0.20

Carbon             1024     22:25         $4.10

DEPT-135         256     13:40          $2.50

COSY          1x256        5:45          $1.05

HSQC          2x256       18:40         $3.42

HMBC         8x128       23:04         $4.23

NOESY       2x256       21:20         $3.91

The number of scans on the 1D's can be increased or decreased to any value; the 2D's typically cannot advantageously be made shorter (i.e. it either won't work at all, or the level of artifacts is likely to be higher). What constitutes enough scans for a particular sample depends on the amount of compound, the amount of solvent (an excess will dilute the sample and make the results worse), and what level of signal to noise you think is appropriate.

I think it is a very good idea to get a proton spectrum of everything submitted on the 500 because it is very indicative of the integrity and concentration of the sample, if nothing else. If you are concerned about the 20 cents, we can shorten another experiment by 16 scans.

The most important thing you can do to get the highest quality results in the shortest time and at the lowest cost is to restrict the amount of solvent used to 0.5-0.6 ml. Less than that may hurt resolution.  Increasing the number of scans on a run is a very cost-inefficient way of making up for an excessively diluted sample, because the signal to noise will only increase with the square root of the number of scans.  If you want to double the S/N, you must make the acquisition 4 times longer (and 4 times as expensive).

You can have an email notification of your sample being run on the 500, with a PDF file of the plot as an attachment.  Let us know if you prefer this.  It is also possible to have the raw data emailed to you, although these attachments are larger than the PDF plot files.


Signup policies:

These vary by instrument. 

The Avance 400 is the most restricted instrument. Prime time runs from 8:30 am to 8 pm weekdays, and signup is not permitted until 4 pm the previous day. From 9:30 am-3pm, no user can reserve, or use (no matter who reserved the time) more than 30 minutes, either in one block or multiple blocks. From 8:30-9:30 am and from 3-8 pm, the maximum is one hour. Users may have both a one hour block and a half hour block in the same day, for a total of 90 minutes maximum.  On Saturday and Sunday, the maximum length is 1 hour between 9 am and 5 pm. In the evening (after 8 on weekdays and after 5 on weekends) there are no restrictions on the length of an experiment and users can sign up as many as seven days in advance.

On the Higuchi and LSRL 400, the weekday limit is 6 hours per person per day between 8 am and 5 pm.  Other times are unrestricted. Since the LSRL instrument is owned by the CMLD, they have priority when they need it.

The Avance 500 runs by automation, so there is no signup per se--drop  off your sample and it's first come, first served.

Hands-on use of the Inova 600 and Avance 800 are restricted to those users approved by the NMR Lab staff. Anyone can get data from these instruments on a service basis on request. Long experiments have priority over short ones at all times. The instrument calendar is kept online at http://kunmr7.chem.ku.edu/cgi-bin/nmr-schedule.cgi.

Scheduling:

The 400's are scheduled on a self service basis using the program "reserve." Instructions are here.
The 600 and 800 calendars are kept online here.  Contact a lab staff member to get on the calendar.

Dave 09/07/07